The Ohio State Highway Patrol is investigating a truck rollover on Ohio 379 a little west of Somerton.
Troopers say the tractor trailer was hauling cows.
Four of the 64 on the truck did die from their injuries.
The surviving cattle were taken to the Barnesville livestock yard.
The accident happened around 3 p.m. Sunday, and took about five hours to clean up.
The driver of the truck was not injured.